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HGH Detection In Blood
Read the Following article on "HGH Detection In Blood".
How is the Human Growth Hormone Detected in Blood?
There are two major ways of detecting the presence of the growth hormone from the body fluids. One is through a urine test and other through blood test. It is a known fact that detection through urine tests is not accurate, as yet. With the advent of Nanotechnology, testing this hormone in urine accurately will also be possible in the future. But this scientific breakthrough will take time to reach the level of implementation. At present, detection by blood test can only be performed.
A methodology for the detection of the recombinant form of this hormone was developed by Simpson H L in 2002, published in the Journal of Medicine and Science in Sports and Exercise. To perform this testing, blood samples are collected from athletes.
Using this technique, insulin-like growth factor-1 and N-terminal extension peptide of procollagen type III marker levels were monitored to detect the Growth Hormone levels. This method was not considered as a perfect one in identifying the hormone levels, but is an effective way of doing the test.
The direct method of testing the occurrence of the recombinant growth hormone is based on the number of isoforms of this synthetic form of the hormone. Natural GH exists in several forms, while synthetic HGH has only one. When the synthetic form of HGH is induced, the quantities of the bio-synthetic HGH will be more in the blood, as compared to that of the natural form. The time period within which it can be detected is 24 to 48 hours, i.e. 24 to 48 hours after the induction. The direct test is not influenced by exercise and depression, as this test is also used for medical purposes.
Another immunoassay was performed in a research study conducted by Kohler M, in 2008, published in the journal of Electrophoresis. This paper dealt with the detection method for distinguishing recombinant GH from the natural form in blood plasma by 2D-PAGE analysis. Here, molecular markers of 22KDa were used, which could identify the recombinant form of the hormone, deposited at that 22KDa weight level. All the other isoforms of natural GH formed bands at different positions.
There are two major ways of detecting the presence of the growth hormone from the body fluids. One is through a urine test and other through blood test. It is a known fact that detection through urine tests is not accurate, as yet. With the advent of Nanotechnology, testing this hormone in urine accurately will also be possible in the future. But this scientific breakthrough will take time to reach the level of implementation. At present, detection by blood test can only be performed.
A methodology for the detection of the recombinant form of this hormone was developed by Simpson H L in 2002, published in the Journal of Medicine and Science in Sports and Exercise. To perform this testing, blood samples are collected from athletes.
Using this technique, insulin-like growth factor-1 and N-terminal extension peptide of procollagen type III marker levels were monitored to detect the Growth Hormone levels. This method was not considered as a perfect one in identifying the hormone levels, but is an effective way of doing the test.
The direct method of testing the occurrence of the recombinant growth hormone is based on the number of isoforms of this synthetic form of the hormone. Natural GH exists in several forms, while synthetic HGH has only one. When the synthetic form of HGH is induced, the quantities of the bio-synthetic HGH will be more in the blood, as compared to that of the natural form. The time period within which it can be detected is 24 to 48 hours, i.e. 24 to 48 hours after the induction. The direct test is not influenced by exercise and depression, as this test is also used for medical purposes.
Another immunoassay was performed in a research study conducted by Kohler M, in 2008, published in the journal of Electrophoresis. This paper dealt with the detection method for distinguishing recombinant GH from the natural form in blood plasma by 2D-PAGE analysis. Here, molecular markers of 22KDa were used, which could identify the recombinant form of the hormone, deposited at that 22KDa weight level. All the other isoforms of natural GH formed bands at different positions.