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HGH Detection
Read the Following article on "HGH Detection".
How is the Human Growth Hormone Detected?
The synthetic form of the growth hormone is used illegally by body builders and sports persons. A supplement of the growth hormone boosts energy levels, enhances lean muscle mass, increases stamina and tolerance, decreases abdominal fat and speeds up recovery from injuries. So, it has been added into the list of banned substances by international Olympic committee, since it gives an unfair competitive advantage to sports personalities who use it. There are certain blood tests and urine tests that are commonly used to detect the presence of bio-synthetic HGH levels.
The technical methodology involved in carrying out these tests include enzyme linked immunosorbent assay. This was published in a journal in 1996 in the research study done by Saugy M, which was published in the Journal of chromatography B, biomedical applications. In this study HGH was given subcutaneously in a dosage of 12 IU/per day. After the treatment, the hormone was found to be excreted 50 to 100 times the basal level, and thereafter, it reaches the usual level after 24 hours. The units of this hormone were also detected using this method in various competitions by keeping control samples which were normal. Growth hormone levels in urine differ based on the extent of practice the athletes do for the competition.
Apart from this test, tests for measuring the level of insulin-like growth factor binding proteins and beta 2-microglobulins are also available to investigate growth hormone doping. Now, there’s another controversy regarding the doping test for GH, since, people have started using GH releasers which serve the same purpose as that of the hormone-injections. The growth hormone releasers yield similar benefits like HGH injections. They enhance lean muscle mass and greatly augment strength and endurance levels, But they are never detected by the tests. This has become another obstruction in the efforts of WADA and USADA in effective developing anti-doping tests.
WADA has taken the decision to produce the antibodies of this hormone on a large scale for the proper implementation of the anti-doping tests. There are other methods like biomarker-based detection methods, but the process of implementing it as an anti-doping test from the research stage is still going on gradually.
A research study was conducted by Heather A Myler, published in January 2010 in the Journal of pharmacological and toxicological methods. According to this study, the circulating concentrations of proteins is detected by specific binding proteins, other components in the fluid and analytes that are endogenous. In the study, they followed pharmacokinetic evaluation of Polyethylene Glycol associated HGH by the implementation of acid dissociation methodology.
This methodology might come into light later to uncover the secrets of effective anti-doping growth hormone detection tests.
The synthetic form of the growth hormone is used illegally by body builders and sports persons. A supplement of the growth hormone boosts energy levels, enhances lean muscle mass, increases stamina and tolerance, decreases abdominal fat and speeds up recovery from injuries. So, it has been added into the list of banned substances by international Olympic committee, since it gives an unfair competitive advantage to sports personalities who use it. There are certain blood tests and urine tests that are commonly used to detect the presence of bio-synthetic HGH levels.
The technical methodology involved in carrying out these tests include enzyme linked immunosorbent assay. This was published in a journal in 1996 in the research study done by Saugy M, which was published in the Journal of chromatography B, biomedical applications. In this study HGH was given subcutaneously in a dosage of 12 IU/per day. After the treatment, the hormone was found to be excreted 50 to 100 times the basal level, and thereafter, it reaches the usual level after 24 hours. The units of this hormone were also detected using this method in various competitions by keeping control samples which were normal. Growth hormone levels in urine differ based on the extent of practice the athletes do for the competition.
Apart from this test, tests for measuring the level of insulin-like growth factor binding proteins and beta 2-microglobulins are also available to investigate growth hormone doping. Now, there’s another controversy regarding the doping test for GH, since, people have started using GH releasers which serve the same purpose as that of the hormone-injections. The growth hormone releasers yield similar benefits like HGH injections. They enhance lean muscle mass and greatly augment strength and endurance levels, But they are never detected by the tests. This has become another obstruction in the efforts of WADA and USADA in effective developing anti-doping tests.
WADA has taken the decision to produce the antibodies of this hormone on a large scale for the proper implementation of the anti-doping tests. There are other methods like biomarker-based detection methods, but the process of implementing it as an anti-doping test from the research stage is still going on gradually.
A research study was conducted by Heather A Myler, published in January 2010 in the Journal of pharmacological and toxicological methods. According to this study, the circulating concentrations of proteins is detected by specific binding proteins, other components in the fluid and analytes that are endogenous. In the study, they followed pharmacokinetic evaluation of Polyethylene Glycol associated HGH by the implementation of acid dissociation methodology.
This methodology might come into light later to uncover the secrets of effective anti-doping growth hormone detection tests.